(1) Using single-cell chromatin accessibility sequencing to characterize CD4+ T cells from murine tissuesFrontiers in ImmunologyOctober 16, 2023Kathrin Luise Braband, Annekathrin Silvia Nedwed, Sara Salome Helbich, Malte Simon, Niklas Beumer, Benedikt Brors, Federico Marini, Michael Delachermale mice are excised and placed in either a 50 ml conical tube or a GentleMACS tube (Miltenyi Biotec #130-096-334) containing VAT digestion buffer ( Table 5 ). The VAT buffer recipe contains a collagenase subtype to

(2) Using combined single-cell gene expression, TCR sequencing and cell surface protein barcoding to characterize and track CD4+ T cell clones from murine tissuesFrontiers in ImmunologyOctober 12, 2023Annekathrin Silvia Nedwed, Sara Salome Helbich, Kathrin Luise Braband, Michael Volkmar, Michael Delacher, Federico Marinifrom the dorsal surface, cut into small pieces, and transferred to a GentleMACS tube (Miltenyi Biotec #130-096-334) containing 10ml of skin digestion buffer (Table 4). We recommend 10ml digestion buffer for 0.5 g of

(3) Generation of mouse hippocampal brain organoids from primary embryonic neural stem cellsSTAR protocolsSeptember 15, 2023Ciarpella F, Zamfir RG, Campanelli A, Pedrotti G, Di Chio M, Bottani E, Decimo I.coverslips,Diapath,061051 Termoblock,FALC, Silicon mold,N/A,N/A Stereomicroscope,N/A,N/A gentleMACS Dissociator,Miltenyi Biotec, gentleMACS C Tubes,Miltenyi Biotec,130-093-237 Cell strainer 40 mm,Corning,352340 Syringe filter 0.22 mm,Sartorius, For

(4) The circular RNA Ataxia Telangiectasia Mutated regulates oxidative stress in smooth muscle cells in expanding abdominal aortic aneurysmsMolecular therapy. Nucleic acidsSeptember 12, 2023Fasolo F, Winski G, Li Z, Wu Z, Winter H, Ritzer J, Glukha N, Roy J, Hultgren R, Pauli J, et al.130-110-203), GentleMACS Dissociator (Miltenyi Biotech, 130-093-235), GentleMACS C tubes (Miltenyi Biotech, 130-096-334), and the 37C_Mulit_G program, all according to the manufacturer’s instructions. The cell suspension

(5) Breast cancer PDxO cultures for drug discovery and functional precision oncologySTAR ProtocolsSeptember 1, 2023Sandra D. Scherer, Ling Zhao, Andrew J. Butterfield, Chieh-Hsiang Yang, Emilio Cortes-Sanchez, Katrin P. Guillen, Bryan E. Welm, Alana L. Welm12-640 10-cm tissue culture plates,Genesee Scientific,cat# 25-202 Gentle MACS C-tubes,Miltenyi Biotec,cat# 130-096-334 PluriStrainer 200 μm,pluriSelect,cat# 43-50200-03 PluriStrainer 300 μm,pluriSelect,cat# 43-50300-03 PluriStrainer

(6) Targeting neoantigens to APC-surface molecules improves the immunogenicity and anti-tumor efficacy of a DNA cancer vaccineFrontiers in ImmunologyAugust 29, 2023Marina Barrio-Calvo, Søren Vester Kofoed, Sofie Cens Holste, Anders Bundgård Sørensen, Nadia Viborg, Jens Vindahl Kringelum, Daniela Kleine-Kohlbrecher, Christian Skjødt Steenmans, Christian Bahne Thygesen, Birgitte Rønø, et al.#10500-064), processed into single cells suspensions by GentleMACS processing (Miltenyi Biotec, C-tubes, #130-096-334 and Dissociator, #130-093-235) and cryopreserved. 2.10 Peptide re-stimulation and intracellular cytokine

(7) Rescue of Alzheimer's disease phenotype in a mouse model by transplantation of wild-type hematopoietic stem and progenitor cellsCell reportsAugust 29, 2023Mishra P, Silva A, Sharma J, Nguyen J, Pizzo DP, Hinz D, Sahoo D, Cherqui S.,Miltenyi Biotec,130-042-401 Miltenyi gentleMACS Octo Dissociator with Heaters,Miltenyi Biotec,130-096-427 Miltenyi gentleMACS C Tubes,Miltenyi Biotec,130-093-237 Biological samples,Biological samples,Biological samples Murine Sca1+

(8) Optimization of differential filtration-based mitochondrial isolation for mitochondrial transplant to cerebral organoidsStem Cell Res TherAugust 15, 2023David F. Bodenstein, Pavel Powlowski, Kassandra A. Zachos, Dana El Soufi El Sabbagh, Hyunjin Jeong, Liliana Attisano, Landon Edgar, Douglas C. Wallace, Ana Cristina Andreazzahomogenization buffer (300 mM sucrose, 10 mM K-HEPES [pH 7.2], and 1 mM K-EGTA [pH 8.0]) and homogenized in gentleMACS C tubes using the gentleMACS Dissociator’s (Miltenyi Biotec, Bergisch Gladbach, North Rhine-Westphalia, Germany)

(9) Correction: Reprogramming anchorage dependency by adherent‑to‑suspension transition promotes metastatic disseminationMolecular cancerAugust 14, 2023Huh HD, Sub Y, Oh J, Kim YE, Lee JY, Kim HR, Lee S, Lee H, Pak S, Amos SE, et al.small pieces of 2–4 mm pieces, then were transferred into the gentleMACS C-tubes (Miltenyi Biotec, 130-096-334) containing the enzyme mix. C-tubes were attached to the sleeve of the gentleMACS Dissociator (Miltenyi Biotec

(10) Single-cell analysis of lizard blastema fibroblasts reveals phagocyte-dependent activation of Hedgehog-responsive chondrogenesisNat CommunAugust 10, 2023Ariel C. Vonk, Xiaofan Zhao, Zheyu Pan, Megan L. Hudnall, Conrad G. Oakes, Gabriela A. Lopez, Sarah C. Hasel-Kolossa, Alexander W. C. Kuncz, Sasha B. Sengelmann, Darian J. Gamble, et al.DPA) were cut to 3–5-mm pieces in length and each piece was cut into 1/8ths. Tail pieces were added to gentleMACS™ C tubes (Miltenyi Biotec, PN: 130-093-237) containing 2.5 mL Dulbecco’s modified Eagle media (DMEM), 100 µL

(11) First-line durvalumab and tremelimumab with chemotherapy in RAS-mutated metastatic colorectal cancer: a phase 1b/2 trialNature medicineAugust 1, 2023Thibaudin M, Fumet JD, Chibaudel B, Bennouna J, Borg C, Martin-Babau J, Cohen R, Fonck M, Taieb J, Limagne E, et al.instructions (130-095-929, Miltenyi Biotec). In brief, tumor was cut into small pieces and transferred into gentleMACS C tubes containing the enzyme mix. The dissociation was performed using the gentleMACS Octo Dissociator with heaters

(12) Prolonged exposure to lung-derived cytokines is associated with inflammatory activation of microglia in patients with COVID-19bioRxivJuly 30, 2023Rogan A Grant, Taylor A. Poor, Lango Sichizya, Estefani Diaz, Joseph I. Bailey, Sahil Soni, Karolina J. Senkow, Xochitl G. Perez-Leonor, Hiam Abdala-Valencia, Ziyan Lu, et al.and 1mg/mL DNAse I (Roche 10104159001) with curved scissors. Chopped tissue was then transferred to gentleMACS C-tubes (Miltenyi 130-093-237) and mixed with 1mL HBSS. Samples were then mechanically dissociated using a gentleMACS Octo Dissociator

(13) Lectin-Seq: A method to profile lectin-microbe interactions in native communitiesScience advancesJuly 28, 2023McPherson RL, Isabella CR, Walker RL, Sergio D, Bae S, Gaca T, Raman S, Nguyen LTT, Wesener DA, Halim M, et al.anaerobically within 24 to 72 hours of collection. In the anaerobic chamber, samples were decanted into sterile gentleMACS C tubes (Miltenyi Biotec, 130-093-237) and topped up to a total volume of 10 ml using 1× PBS + 40% glycerol

(14) High-fat-diet-associated intestinal microbiota exacerbates psoriasis-like inflammation by enhancing systemic γδ T cell IL-17 productionCell reportsJuly 25, 2023Sonomoto K, Song R, Eriksson D, Hahn AM, Meng X, Lyu P, Cao S, Liu N, Taudte RV, Wirtz S, et al.flow cytometry buffer (DPBS +2% FBS +20 mM EDTA) and the resulting suspension was then transferred into gentleMACS C tubes (Miltenyi Biotec). Tissue dissociation was performed by using program B_01 3 times in the gentleMACS

(15) A Th17 cell-intrinsic glutathione/mitochondrial-IL-22 axis protects against intestinal inflammationbioRxivJuly 13, 2023Lynn Bonetti, Veronika Horkova, Joseph Longworth, Luana Guerra, Henry Kurniawan, Davide G. Franchina, Leticia Soriano-Baguet, Melanie Grusdat, Sabine Spath, Eric Koncina, et al.rotation. Again, tissues were recovered in a 100µm strainer and transferred into a C Tube (Miltenyi Biotec; 130–096-334) containing HBSS with Ca2+, Mg2+ (Westburg) and an enzyme mix (enzyme D, enzyme R, enzyme A) prepared

(16) Intranasal influenza-vectored COVID-19 vaccine restrains the SARS-CoV-2 inflammatory response in hamstersNature CommunicationsJuly 11, 2023Liang Zhang, Yao Jiang, Jinhang He, Junyu Chen, Ruoyao Qi, Lunzhi Yuan, Tiange Shao, Hui Zhao, Congjie Chen, Yaode Chen, et al.All cells were counted using CountStar software. Lung Lungs were cut into 0.5-cm pieces, placed in gentleMACS C tubes (Miltenyi) containing collagenase type IV (Gibco) and DNase I (Roche) in PBS containing 2% FBS, and

(17) The Antitumor Activities of Anti-CD47 Antibodies Require Fc-FcγR interactionsbioRxivJuly 5, 2023Juan C. Osorio, Patrick Smith, David A. Knorr, Jeffrey V. Ravetchinoculation. Tissue processing Tumors were dissected and cut into small pieces and transferred to gentleMACS C tubes (Miltenyi Biotec) containing enzyme mix for tough tumors (Catalog 130-096-730m Miltenyi Biotec) in Dulbecco’s

(18) In silico-in vitro modeling to uncover cues involved in establishing microglia identity: TGF-β3 and laminin can drive microglia signature gene expressionFrontiers in Cellular NeuroscienceJune 26, 2023Raissa Timmerman, Ella Alwine Zuiderwijk-Sick, Wia Baron, Jeffrey John Bajramovicdepleted of meninges and blood vessels manually. Tissue was chopped into cubes of less than 2 mm2 by using gentleMACS™ C tubes (Miltenyi Biotec) and incubated at 37°C for 20 min in PBS containing 0.25% (w/v) trypsin (Gibco, Life Technologies

(19) Reconstructed human intestinal comet assay, a possible alternative in vitro model for genotoxicity assessmentMutagenesisJune 20, 2023Christopher Owen Hughes, Hui Kheng Lim, Joseph Choon Wee Wee Tan, David Ian Leavesley, Benjamin Paul Chapman Chapman Smithwith the use of forceps. Detached tissues recovered into in cell recovery solution were placed into gentleMACS C-tubes (Miltenyi Biotech, Bergisch Gladbach, Germany) containing the enzyme mix described in the human tumour

(20) Single cell profiling of female breast fibroadenoma reveals distinct epithelial cell compositions and therapeutic targetsNature communicationsJune 16, 2023Chen Z, Zhang Y, Li W, Gao C, Huang F, Cheng L, Jin M, Xu X, Huang J.containing 1 mg/mL collagenase (Sigma-Aldrich, Cat.# C9407) in gentleMACS c-tubes (Miltenyi Biotec, 130-096-334). Samples were dissociated completely by a gentleMACS octo Dissociator with Heaters (Miltenyi Biotec,

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C Tubes

gentleMACS C Tubes (4x 25) from Miltenyi Biotec

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gentleMACS C Tubes (4x 25) from Miltenyi Biotec

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